Journal: Stem Cells Translational Medicine

Article Title: Chondroitin Sulfate Glycosaminoglycan Scaffolds for Cell and Recombinant Protein‐Based Bone Regeneration

doi: 10.1002/sctm.18-0141

Figure Lengend Snippet: Efficient transduction of mesenchymal stem cells with a lentiviral vector at 10 MOI. (A): Images of uMSC over 72 hours after transducing with prEF1a‐RFP at 10 and 50 MOI under phase contrast and RFP filter to evaluate transduction efficiency. Scale bars indicate 200 μm. N = 15. (B): Mean BMP‐2 expression in uMSCs and bmMSCs at 48, 72, 96, and 120 hours after transducing with prEF1a‐BMP2 at 10 and 50 MOI, comparing expression over cell type and MOI within each time point. Transductions at 0 MOI did not result in BMP‐2 expression detectable by BMP‐2 enzyme‐linked immunosorbent assay. Groups with differing letters are significantly different from each other within a time point at p < .05. Error bars indicate standard error. Two‐way analysis of variance with Tukey's multiple comparison test ( n = 5–6). Abbreviations: bmMSC, bone marrow mesenchymal stem cell; BMP‐2, bone morphogenetic protein 2; MOI, moment of inertia; uMSC, umbilical mesenchymal stem cell.

Article Snippet: Because of the differences in MSC behavior as a result of tissue source, both human umbilical (uMSC) and bone marrow MSCs (bmMSC; Lifeline Cell Technology, Frederick MD, Sciencell, Carlsbad, CA; one donor each) were used .

Techniques: Transduction, Plasmid Preparation, Expressing, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Regenerative Therapy

Article Title: In-process evaluation of culture errors using morphology-based image analysis

doi: 10.1016/j.reth.2018.06.001

Figure Lengend Snippet: Morphology-based analysis of growth rate performance. (A) Growth rate performance among the 21 lots of MSCs used in this study. (B) Representative phase contrast images of MSC lots (Lot 6 and 5) and images of morphological transition analysis. Scale bar = 200 μm. All images were prepared with the same scale. (C) Representative image of morphological transition analysis applied to group A (good growth lots) and group B (bad growth lots). (D) Prediction performance of growth rate-prediction models examining the time-course window effect.

Article Snippet: Nine lots of human bone-marrow derived MSCs were purchased from Lonza Japan, Ltd. (Tokyo, Japan) and 1 lot was purchased from Lifeline Cell Technology (Frederick, MD, USA).

Techniques:

Journal: Regenerative Therapy

Article Title: In-process evaluation of culture errors using morphology-based image analysis

doi: 10.1016/j.reth.2018.06.001

Figure Lengend Snippet: Morphology-based analysis of errors in cell culture. (A) Representative phase contrast images of MSCs under 5 types of culture conditions (Standard and 4 types of intentional errors). Scale bar = 200 μm. All images were prepared with the same scale. (B) Representative visualization image of morphological transition analysis applied to the 5 culture conditions. (C) Prediction performances of culture condition discrimination models examining the time-course window effect.

Article Snippet: Nine lots of human bone-marrow derived MSCs were purchased from Lonza Japan, Ltd. (Tokyo, Japan) and 1 lot was purchased from Lifeline Cell Technology (Frederick, MD, USA).

Techniques: Cell Culture